Spectrophotometry is a technique that has the ability to evaluate and measure how much light can be absorbed by a substance, either solid or liquid, this technique seeks to measure the luminous intensity of the sample when it passes through a beam of light. Spectrometry is based on the Beer-Lambert law, which explains that there is an exponential interaction between the transmission of light by means of a substance and the concentration of the same, this foundation allows it to be used in different medical and scientific areas.
Spectrometry has several applications in different areas, as well as biology, medicine and chemistry, among the functions of this technique is to analyze in a quantitative and qualitative way the test or unknown samples in research laboratories, determine the concentration of samples and identify components thereof. In order to perform this important technique, it is necessary to use spectrophotometers. Below we will explain how they work and how important they are within the laboratories.
Spectrophotometers
If you need to perform spectrophotometry in the laboratory, it is essential that you have a spectrophotometer, which is nothing more than a team responsible for measuring the absorbance of a test sample, in order to know what the concentration of the components deposited in it is. Its operation is very simple, based on the comparison of a test sample with a standard sample, which are crossed with a beam of light.
Within the laboratories there are several types of spectrophotometers, the choice of them depends on the type of sample to be analyzed. There are atomic absorption spectrophotometers and molecular absorption spectrophotometers, although they are different, both fulfill the fundamental function of a spectrophotometer, which is the determination of sample concentrations. The characteristics of the different types of spectrophotometers are shown below:
- Atomic absorption spectrophotometer: it is a highly efficient equipment, it allows the determination of the concentrations of the components of a test sample at very specific levels, this allows it to be widely used for the identification of elements of different types, whether clinical, industrial or environmental samples.
- Molecular absorption spectrophotometer: also known as visible ultraviolet molecular absorption spectrophotometer or UV-VIS. This is based on the amount of visible ultraviolet radiation that is absorbed by certain molecules, so certain compounds can be identified.
Spectrophotometer technology
The main element of any spectrophotometer is a specialized light lamp. The light from this lamp is guided through a connector that selects and separates the specific wavelength that will then be passed to the test sample. Subsequently, the intensity of light that came out after passing through the sample is calculated, in relation to the intensity of light that initially affected, in this way you can know the procedure, which depends on how concentrated the substance is.
In general, it can be said that the technology of its operation is based on the illumination of a sample, in order to know the amount of light that is reflected. The basis of this technique allows it to be used in areas such as chemical engineering, biochemistry, biology, physics, engineering, in clinics, and in any laboratory where chemical analysis is necessary. For example, in clinics they are necessary because blood and tissue can be examined, in some industries, such as optics, used to measure the processing of solid substances.
Kalstein spectrophotometers
Kalstein has in its catalog a great diversity of laboratory equipment and medical instruments, being MANUFACTURERS of these, we assure you that any equipment you acquire will be of high quality and long durability. If you want to make the PURCHASE of a spectrophotometer, we have a wide range at the best PRICES, we have hand, table, portable, double beam, single beam and ultraviolet light equipment, if you need to make specific analyzes. Of course, you will be advised at all times by our team of experts, who will help you choose the option that best suits the analyzes you perform.
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