What technology does an ELISA microplate reader use?
An ELISA microplate reader is a laboratory instrument that is responsible for detecting and processing biological and chemical data by absorbance (ELISA, enzymatic activity and quantification of nucleic acids and proteins), luminescence and fluorescence detection modes, including intensity, TRF and polarization according to the model. They are useful laboratory tools used in drug discovery, research, immunologic testing, hormone detection, bioassay validation, and biopharmaceutical manufacturing.
Is an Elisa Microplate Washer Important?
The importance of the Elisa technique, has to do with the detection of diseases until quantification by immunoabsorption assays that linked to enzymes, detect color changes, and allow the measurement of antigen in the sample, using spectrophotometry.
What is an ELISA reader used for?
An ELISA reader is a specialized spectrophotometer used to read the results of this technique, which is used to determine the presence of specific antibodies or antigens present in a sample. These laboratory equipment allows analyzing 96 wells or more with samples, so their use is very favorable to the laboratories, reducing reagents and samples used which leads to better performance.
Importance in the manufacture of drugs against lupus with the use of the Analytical Balance
Lupus is an autoimmune disease in which defense cells of the immune system attack the body’s own cellular structures and cause inflammatory changes in various organs of the body. Today, despite advances in medicine, it is not known why this disease is triggered. According to autoimmune theory, lupus is caused by the body fighting itself. However, triggers such as stress, viruses, active hormonal phases, UV light, and some drugs can be mentioned.
The use of the Analytical Balance to develop vaccines against Covid-19
The health event that has marked the population globally has undoubtedly been the COVID-19 pandemic, and the virus that has caused it, SARS-Cov-2 and its variants. The disease can complicate people’s respiratory system to the point of collapse, sometimes causing death.
What technology do real-time thermocyclers use?
A real-time thermo-cycler is a device that simultaneously accurately regulates thermal cycles so that the DNA chains are multiplied by the polymerase chain reaction (PCR), while incorporating a technology that allows the reaction to be tracked. To achieve the latter purpose, the thermocycler is incorporated with a fluorometer, which is made up of an excitation source, absorption filters, a radiation detector and the electronics needed to process electrical signals and transform them into digital signals suitable for computer equipment.
What is a thermal cycler?
The thermal cycler is a device that allows the polymerase chain reaction (PCR) efficiently and quickly; by means of the automatic and cyclical realization of the temperature changes that are required for the amplification of a deoxyribonucleic acid (DNA) chain; from a thermostable enzyme.
Thermocycler: How many types are there?
The thermocycler is a laboratory molecular biology team that allows the polymerase chain reaction (PCR) efficiently and quickly, by performing automatic and cyclic temperature changes required for the amplification of a deoxyribonucleic acid (DNA) chain, from a thermostable enzyme. The PCR technique allows a small amount of DNA molecules to be amplified many times, exponentially.
Thermal cyclers: Care and recommendations
Thermal cyclers or thermal sequencers are laboratory equipment, which allow to carry out the amplification of DNA molecules using the PCR (Polymerase Chain Reaction) technique, through cyclical changes in temperatures that in turn generate amplification. of strands of this nucleic acid. This equipment is used for qualitative amplifications or even to quantify the amount of amplified DNA, since it is designed to change the reaction temperature every few minutes, making denaturation and DNA synthesis possible.
Real-time thermocycler: How does it work?
A real-time thermocycler is a molecular biology equipment widely used in the laboratories of this specialty. These devices carry out the polymerase chain reaction in real time (qPCR), that is to say they allow carrying out the PCR and at the same time they can absolutely quantify the product of the amplification.